HPLC Columns and Cartridges
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TSKgel™ ODS-120T HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ ODS-120T columns use a 150 Å pore size silica base support. The silica′s exclusion limit of 10,000 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and small molecular weight compounds in organic and environmental samples.
| Pore Size | 150 Å |
|---|---|
| Solvents | 0 to 100% organic solvent |
| Stationary Phase | C18 (octadecyl) |
| Temperature | 10°C to 50°C |
| Phase | Reversed Phase |
| Surface Area | 200 m2/g |
| Particle Size | 5 μm |
| Flow Rate | 1.2 mL/min. |
| Carbon Load | 22% |
| Matrix | Silica Particle Platform |
| Diameter (Metric) | 4.6 mm |
| pH | 2.0 - 7.5 |
| Product Line | TSKgel |
| USP Type | L1 |
TSKgel™ Phenyl-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
The most hydrophobic among the three TSKgel™ HIC columns, TSKgel™ Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules.
TSKgel™ Amide-80 Guard Columns, 2 μm Particle Size, Sold by MilliporeSigma™ Supelco™
For use with TSKgel Amide-80 Columns, 5 μm. TSKgel Guard Columns are stand-alone and do not require separate holders.
| Stationary Phase | Carbamoyl |
|---|---|
| For Use With (Equipment) | TSKgel™ Amide-80 Columns (5 μm) |
| pH | 2 to 7.5 |
| Technique | Hydrophilic Interaction (HILIC) |
| Surface Area | 450 m2/g |
| Product Line | TSKgel |
| USP Type | L68 |
TSKgel™ SuperHZ1000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel SuperHZ1000 Columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 1,000 Da.
| Pore Size | 15 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Polystyrene-divinylbenzene |
| Temperature | 60°C |
| Matrix | Polystyrene-divinylbenzene Phase/Polymer Particle Platform |
| Length (Metric) | 15 cm |
| Technique | SEC |
| Max. Pressure | 55 bar |
| Product Line | TSKgel |
TSKgel™ SuperHZ Guard Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ Guard Columns are stand-alone and do not require separate holders. For use with TSKgel SuperHZ1000-4000 and HZM-N & -M Columns, 3 μm.
| Pore Size | Mixed |
|---|---|
| Particle Size | 3 μm |
| Flow Rate | 0.4 mL/min |
| Stationary Phase | Polystyrene-divinylbenzene |
| Packing Material | Polymer Particle Platform |
| Diameter (Metric) Inner | 4.6 mm |
| Technique | Size Exclusion (SEC) |
| Temperature (Metric) Maximum | 80°C |
| Product Line | TSKgel |
TSKgel™ SuperSW3000 HPLC Column, 4 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Featuring ultra-efficient 4 μm particles, TSKgel SuperSW3000 provides higher efficiency than 5 μm TSKgel G2000SWxl Columns. The 125 Å pore size results in a fractionation range up to 150,000 Da for globular proteins.
| Shipping Condition | Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M Phosphate Buffer |
|---|---|
| Pore Size | 250 Å |
| Particle Size | 4 μm |
| Solvents | 0-100% Aqueous Soluble Organic Solvents |
| Stationary Phase | Diol |
| Temperature | 10°C to 30°C |
| Matrix | Diol Phase/Silica Particle Platform |
| Length (Metric) | 30 cm |
| pH | 2.5 to 7.5 |
| Technique | SEC |
| Max. Pressure | 120 bar |
| Product Line | TSKgel |
| USP Type | L20, L59 |
MilliporeSigma™ Supelco™ Ascentis™ C18 Supelguard™ Guard Cartridge, 5 μm Particle Size
Ascentis™ C18 is an extremely stable and reliable HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 5 μm |
| Type | Fused-Core HPLC Guard Cartridge |
| Phase | Reversed Phase |
| Length (Metric) | 2 cm |
| Endcapped | Yes |
| pH | 2 to 8 |
| Quantity | 2/Pk. |
| For Use With (Application) | HPLC |
| Surface Area | 450 m2/g |
| Product Line | Ascentis Supelguard |
MilliporeSigma™ Supelco™ apHera™ NH2 HPLC Column, 5 μm Particle Size
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides.
| Pore Size | 300 Å |
|---|---|
| Particle Size | 5 μm |
| Stationary Phase | Amino (NH2) |
| Phase | Normal Phase and HILIC |
| Matrix | Amino (NH2) Phase |
| Product Line | apHera |
TSKgel™ G3000SW HPLC Column, 13 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ G3000SW SEC columns provide excellent separation of proteins and other biomolecules with molecular weights up to 500,000 Da.
| Particle Size | 13 μm |
|---|---|
| Solvents | 0 to 100% aqueous soluble organic solvents |
| Flow Rate | 8 mL/min. |
| Stationary Phase | Diol |
| Temperature | 10°C to 30°C |
| Phase | Size Exclusion |
| Matrix | Silica Particle Platform |
| Diameter (Metric) | 21.5 mm |
| pH | 2.5 - 7.5 |
| Product Line | TSKgel |
| USP Type | L20, L59 |
TSKgel™ SuperH2500 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Packed with rigid, porous polymer beads and used for the non-aqueous separation of polymers with molecular weights less than 20,000 Da.
TSKgel™ HHR Guard Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
All TSKgel HHR-type columns are shipped in THF, which can be exchanged for a broad range of organic solvents.
TSKgel™ Protein C4-300 Guard Cartridge, Sold by MilliporeSigma™ Supelco™
Addition to the extensive line of Reversed Phase columns from Tosoh Bioscience
| Particle Size | 3 μm |
|---|---|
| Flow Rate | 1 mL/min |
| Stationary Phase | C4 (butyl) |
| Matrix | Spherical silica |
| For Use With (Equipment) | L26 Per USP |
| pH | 1.5 - 7.5 |
| Technique | Reversed phase |
| Temperature (Metric) Maximum | 10°C to 50°C |
| Surface Area | 100 m2/g |
TSKgel™ Amide-80 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products.
| Pore Size | 100 Å |
|---|---|
| Particle Size | 3 μm |
| Stationary Phase | Carbamoyl |
| Temperature | 10°C to 50°C |
| Max. Pressure | 200 bar |
| Product Line | TSKgel |
| USP Type | L68 |
TSKgel™ SuperSW mAb HR HPLC Column, 4 μm Particle Size, Sold by MilliporeSigma™ Supelco™
TSKgel™ superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).
| Pore Size | 250 Å |
|---|---|
| Particle Size | 4 μm |
| Solvents | 0 to 100% aqueous soluble organic solvents |
| Stationary Phase | Diol |
| Temperature | 10°C to 30°C |
| Phase | Size Exclusion |
| Matrix | Silica Particle Platform |
| pH | 2.5 - 7.5 |
| Material | Stainless Steel |
| Product Line | TSKgel |
| USP Type | L20, L59 |
TSKgel™ SCX (Na+) HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™
Packed with 5 mm porous, spherical particles which are surface modified with a strong (sulfonic acid) cation exchanger in the sodium form. Columns are optimized for the separation and analysis of organic acids, saccharides, and alcohols.